2EWB

The crystal structure of Bovine Lens Leucine Aminopeptidase in complex with zofenoprilat

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 63 2 2
Unit cell lengths	128.940, 128.940, 119.790
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.850
Rwork	0.156
R-free	0.17700
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1lam
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.920
High resolution limit [Å]	1.850	1.850
Number of reflections	50083
<I/σ(I)>	22	9.2
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	TRIS/HCl pH 7.8, MPD, Zinc chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298.