2EW1
Crystal Structure of Rab30 in complex with a GTP analogue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.002, 57.955, 82.921 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.510 - 2.000 |
| R-factor | 0.23662 |
| Rwork | 0.234 |
| R-free | 0.28423 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1YU9.pdb |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.116 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 14546 | |
| <I/σ(I)> | 47.2 | 10.13 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.6 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 30% PEG 4K, 0.2M MgCl2, 0.1 M Tris-HCl 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
