2EW0
X-ray Crystal Structure of Protein Q6FF54 from Acinetobacter sp. ADP1. Northeast Structural Genomics Consortium Target AsR1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 |
| Unit cell lengths | 105.848, 105.848, 35.039 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.220 - 1.400 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.21700 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.053 | 0.374 |
| Number of reflections | 85882 | |
| <I/σ(I)> | 72.8 | |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 24.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 2M (NH4)2SO4, 2% PEG400, 0.1M MES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
