2EVX

Crystal structure of pumpkin seed globulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-30
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0
Spacegroup name	F 2 3
Unit cell lengths	189.290, 189.290, 189.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.970 - 2.600
R-factor	0.205
Rwork	0.205
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.079	0.374
Number of reflections	17371
Completeness [%]	100.0	100
Redundancy	18.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP