2EVH
Structure of a Ndt80-DNA complex (MSE mutant mA7G)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2001-12-08 |
| Detector | SBC-2 |
| Wavelength(s) | 1.0093 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.534, 78.441, 161.334 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.800 - 1.989 |
| R-factor | 0.18 |
| Rwork | 0.179 |
| R-free | 0.21200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | PDB 1MNN - MINUS DNA AT SEQUENCE CHANGES AND 1 BASEPAIR ADJACENT TO THOSE CHANGES |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.331 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.800 | 2.120 |
| High resolution limit [Å] | 1.989 | 1.989 |
| Number of reflections | 29735 | |
| Completeness [%] | 97.2 | 93.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 30% PEG 400, 50 mM bis-tris-propane pH 7.0, 100 mM NaCl, 50 mM CaCl2, and 5 mM DTT. 1:1 Molar ratio protein:DNA, protein at 15 mg/ml, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | bis-tris-propane | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | CaCl2 | ||
| 5 | 1 | 1 | DTT | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | PEG 400 | ||
| 8 | 1 | 2 | NaCl | ||
| 9 | 1 | 2 | CaCl2 |
