2EVG
Structure of a Ndt80-DNA complex (MSE mutant mA7T)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2003-07-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.072158 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.432, 78.781, 162.671 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.120 - 1.550 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.18400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | PDB 1MNN - MINUS DNA AT SEQUENCE CHANGES AND 1 BASEPAIR ADJACENT TO THOSE CHANGES |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.397 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 32.320 | 32.530 | 1.630 |
| High resolution limit [Å] | 1.550 | 4.900 | 1.550 |
| Rmerge | 0.053 | 0.035 | 0.283 |
| Number of reflections | 64927 | ||
| <I/σ(I)> | 8.1 | 15.3 | 2.5 |
| Completeness [%] | 98.6 | 99.2 | 90.8 |
| Redundancy | 4.9 | 4.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 30% PEG 400, 50 mM bis-tris-propane pH 7.0, 100 mM NaCl, 50 mM CaCl2, and 1 mM DTT. 1:1 Molar ratio protein:DNA, protein at 10mg/ml, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | bis-tris-propane | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | CaCl2 | ||
| 5 | 1 | 1 | DTT | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | PEG 400 | ||
| 8 | 1 | 2 | NaCl | ||
| 9 | 1 | 2 | CaCl2 |
