2EVD
Crystal structure of human Glycolipid Transfer Protein complexed with 12:0 Lactosylceramide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-12-09 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 75.592, 49.360, 68.632 |
Unit cell angles | 90.00, 122.38, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.181 |
Rwork | 0.178 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2eum |
RMSD bond length | 0.014 |
RMSD bond angle | 1.612 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.058 | 0.602 |
Number of reflections | 14549 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.2 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.5 | 293 | 15-20% PEG 3350 or 8000, 50 mM potassium phosphate, pH 4.5, VAPOR DIFFUSION, temperature 293K |