2EV3
Structure of Rv1264N, the regulatory domain of the mycobacterial adenylyl cylcase Rv1264, at pH 5.3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.934 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.403, 52.976, 89.399 |
Unit cell angles | 90.00, 133.73, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.680 |
R-factor | 0.23084 |
Rwork | 0.229 |
R-free | 0.26517 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.957 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.820 |
High resolution limit [Å] | 2.680 | 2.680 |
Rmerge | 0.077 | 0.360 |
Number of reflections | 11593 | |
<I/σ(I)> | 6.4 | 1.9 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 5.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 291 | PEG 400, sodium acetate, calcium acetate, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |