2EV3
Structure of Rv1264N, the regulatory domain of the mycobacterial adenylyl cylcase Rv1264, at pH 5.3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.403, 52.976, 89.399 |
| Unit cell angles | 90.00, 133.73, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.680 |
| R-factor | 0.23084 |
| Rwork | 0.229 |
| R-free | 0.26517 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.957 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.820 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.077 | 0.360 |
| Number of reflections | 11593 | |
| <I/σ(I)> | 6.4 | 1.9 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 5.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 291 | PEG 400, sodium acetate, calcium acetate, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
