2EV2
Structure of Rv1264N, the regulatory domain of the mycobacterial adenylyl cylcase Rv1264, at pH 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.105, 54.096, 89.341 |
| Unit cell angles | 90.00, 132.05, 90.00 |
Refinement procedure
| Resolution | 14.890 - 2.350 |
| R-factor | 0.2134 |
| Rwork | 0.211 |
| R-free | 0.25693 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.395 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 ((MOLREP)) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.064 | 0.405 |
| Number of reflections | 18902 | |
| <I/σ(I)> | 13.8 | 1.6 |
| Completeness [%] | 99.3 | 97.1 |
| Redundancy | 4.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | ammonium sulphate, CAPS, lithium sulphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
