2EV0
Bacillus subtilis manganese transport regulator (MNTR) bound to cadmium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-22 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.900, 46.200, 74.900 |
| Unit cell angles | 90.00, 93.00, 90.00 |
Refinement procedure
| Resolution | 39.950 - 1.650 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1on1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.048 | 0.195 |
| Number of reflections | 37469 | |
| Completeness [%] | 92.6 | 90.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG 400, 0.15 M LITHIUM SULFATE, pH 8.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
