2EUI

Crystal structure of a probable acetyltransferase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9805, 0.9802, 0.9400
Spacegroup name	P 41
Unit cell lengths	115.100, 115.100, 66.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.800
Rwork	0.227
R-free	0.28300
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.324
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.139	0.473
Number of reflections	21842
<I/σ(I)>	6.6
Completeness [%]	100.0	99.7
Redundancy	13.6	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	25% PEG 3350, 0.2M Magnesium Chloride, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K