2EU8
Crystal structure of a thermostable mutant of Bacillus subtilis Adenylate Kinase (Q199R)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.690, 75.140, 77.350 |
| Unit cell angles | 90.00, 98.13, 90.00 |
Refinement procedure
| Resolution | 37.570 - 1.800 |
| R-factor | 0.2289 |
| Rwork | 0.187 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zin |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.235 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK (8.0SSI) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.570 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.036 | 0.096 |
| Number of reflections | 48747 | |
| <I/σ(I)> | 21.7 | 8.7 |
| Completeness [%] | 94.0 | 80.4 |
| Redundancy | 3.84 | 3.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293.14 | 0.75mM protein, PEG1500, Calcium Chloride, Ches, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.14K |
