2ETH
Crystal structure of a marr-like transcriptional regulator (tm0816) from thermotoga maritima at 2.50 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-25 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837, 0.97932 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 98.852, 58.439, 80.432 |
Unit cell angles | 90.00, 125.91, 90.00 |
Refinement procedure
Resolution | 45.030 - 2.300 |
Rwork | 0.227 |
R-free | 0.26400 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.562 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.190 | 47.190 | 2.360 |
High resolution limit [Å] | 2.300 | 10.290 | 2.300 |
Rmerge | 0.058 | 0.021 | 0.637 |
Number of reflections | 14519 | ||
<I/σ(I)> | 9.6 | 27.6 | 1.2 |
Completeness [%] | 87.3 | ||
Redundancy | 3.5 | 3.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 277 | 2.0M (NH4)2SO4, 0.2M NaCl, 0.1M Cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |