2ETE
Recombinant oxalate oxidase in complex with glycolate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 250.434, 250.434, 250.434 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.750 |
| R-factor | 0.1821 |
| Rwork | 0.181 |
| R-free | 0.19367 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fi2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.207 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.073 | 0.284 |
| Number of reflections | 67848 | |
| <I/σ(I)> | 33 | 10 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14 | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 10% PEG4000, 0.1M sodium acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
