2ETD
Crystal structure of a lema protein (tm0961) from thermotoga maritima msb8 at 2.28 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0163, 0.9797, 0.9796 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 80.454, 85.259, 53.397 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.700 - 2.280 |
| Rwork | 0.216 |
| R-free | 0.27000 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.362 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 4.840 | 2.250 |
| Rmerge | 0.067 | 0.041 | 0.332 |
| Number of reflections | 8126 | ||
| Completeness [%] | 90.1 | ||
| Redundancy | 3.5 | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 6.3 | 277 | 0.2M NH4NO3, 20.0% PEG-3350, No Buffer pH 6.3 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
