2ETB
Crystal structure of the ankyrin repeat domain of TRPV2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-25 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.954, 57.476, 139.433 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| R-factor | 0.18912 |
| Rwork | 0.185 |
| R-free | 0.22194 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eta |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.424 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.057 | 0.443 |
| Number of reflections | 44271 | |
| <I/σ(I)> | 11.2 | 2.5 |
| Completeness [%] | 99.7 | 78.1 |
| Redundancy | 4.38 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.17mM protein, 1.2M lithium acetate, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
