2ESW

Atomic structure of the N-terminal SH3 domain of mouse beta PIX,p21-activated kinase (PAK)-interacting exchange factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-05-23
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	88.287, 88.287, 40.558
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.010
R-factor	0.254
Rwork	0.232
R-free	0.25400
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.206
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.080
High resolution limit [Å]	2.010	2.010
Number of reflections	22511
Completeness [%]		98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.1M Hepes Na, 1.45M Lithium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K