2ESR
conserved hypothetical protein- streptococcus pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Detector technology | CCD |
| Collection date | 2005-09-19 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.170, 73.586, 76.916 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.810 - 1.800 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.24900 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.098 | 0.386 |
| Number of reflections | 68188 | |
| <I/σ(I)> | 20 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.9 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 273 | 20% PEG3350 0.3M AmSulfate Cryo: Glucose, pH 7.0, VAPOR DIFFUSION, temperature 273K |
