2ESN

The crystal structure of probable transcriptional regulator PA0477 from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-21
Detector	CUSTOM-MADE
Wavelength(s)	0.978959
Spacegroup name	P 21 21 2
Unit cell lengths	97.035, 171.209, 86.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.19549
Rwork	0.194
R-free	0.25117
Structure solution method	SAD
RMSD bond length	0.022
RMSD bond angle	1.919
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	79899
<I/σ(I)>	35	2.6
Completeness [%]	97.1	94.47
Redundancy	7.2	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	10% PEG6K. 5%MPD, 0.1MHepes-Na pH7.5 20mM imidazole, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	10% PEG6K. 5%MPD, 0.1MHepes-Na pH7.5 20mM imidazole, VAPOR DIFFUSION, HANGING DROP, temperature 298K