2ES2
Crystal Structure Analysis of the Bacillus Subtilis Cold Shock Protein Bs-CspB in Complex with Hexathymidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-10-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91840 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 49.030, 53.170, 76.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.000 - 1.780 |
| R-factor | 0.19 |
| Rwork | 0.189 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB-ENTRY 1CSP |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.894 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 2.000 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.065 | 0.426 |
| Number of reflections | 9870 | 2773 |
| <I/σ(I)> | 12.63 | 3.45 |
| Completeness [%] | 98.5 | 96.3 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2M calcium acetate 18% (w/v) PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
