2ERY

The crystal structure of the Ras related protein RRas2 (RRAS2) in the GDP bound state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-01
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	62.191, 72.115, 82.073
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.700
R-factor	0.198
Rwork	0.196
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 1X1R.PDB
RMSD bond length	0.009
RMSD bond angle	1.352
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	40966
Completeness [%]	98.9	92.7
Redundancy	5.8	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5	277	8 % PEG3350, 0.005 M MgCl2, 0.005 M CoCl2, 0.1 M Bis Tris, 0.1 M Mg(Formate)2, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 5.50