2ERE
Crystal Structure of a Leu3 DNA-binding domain complexed with a 15mer DNA duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2000-06-08 |
Wavelength(s) | 0.9213 |
Spacegroup name | P 62 |
Unit cell lengths | 66.565, 66.565, 122.768 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.120 - 3.000 |
R-factor | 0.277 |
Rwork | 0.277 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | One biological unit of pdb entry 2ER8 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.200 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.000 | |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 6131 | |
<I/σ(I)> | 4.8 | 3.2 |
Completeness [%] | 98.3 | 98.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 50 mM Cacodylate, 200 mM NH4OAc, 10 mM CaCl2, 5% PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Cacodylate | ||
10 | 1 | 2 | H2O | ||
2 | 1 | 1 | PEG 4000 | ||
3 | 1 | 1 | NH4OAc | ||
4 | 1 | 1 | CaCl2 | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | Cacodylate | ||
7 | 1 | 2 | NH4OAc | ||
8 | 1 | 2 | CaCl2 | ||
9 | 1 | 2 | PEG 4000 |