2EPO
N-acetyl-B-D-glucosaminidase (GCNA) from Streptococcus gordonii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.008 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 110.040, 112.490, 103.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.880 - 1.560 |
| R-factor | 0.21237 |
| Rwork | 0.210 |
| R-free | 0.24926 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Selenium methionine structure |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.268 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.880 | 1.600 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.093 | 0.430 |
| Number of reflections | 170106 | |
| <I/σ(I)> | 11.7 | 4.36 |
| Completeness [%] | 96.8 | 77.6 |
| Redundancy | 3.6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100mM sodium citrate, 200mM ammonium acetate, 24%(v/v) PEG4000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
