2EPL
N-acetyl-B-D-glucosaminidase (GCNA) from Streptococcus gordonii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97928 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 123.561, 124.535, 147.793 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.390 - 1.400 |
| R-factor | 0.18699 |
| Rwork | 0.186 |
| R-free | 0.19905 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | accompanying SeMet structure |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.304 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.090 | 0.500 |
| Number of reflections | 221868 | |
| <I/σ(I)> | 17.8 | 2.53 |
| Completeness [%] | 99.9 | 99.39 |
| Redundancy | 9.8 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 293 | 100mM Tris, 2M ammonium sulphate, 2 %(v/v) PEG400, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
