2EPG
Crystal structure of TTHA1785
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-07 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97884, 0.97935, 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.689, 66.685, 116.988 |
| Unit cell angles | 90.00, 101.21, 90.00 |
Refinement procedure
| Resolution | 38.630 - 2.100 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.23800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.084 | 0.619 |
| Number of reflections | 56468 | |
| <I/σ(I)> | 21.3 | 2.32 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 5.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20mM Tris (pH 8.0), 150mM NaCl, 1mM DTT, 20% PEG 3350, 0.2M MgSO4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
