2EO8
Crystal structure of a mutant pyrrolidone carboxyl peptidase (A199P) from P. furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-23 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.636, 104.423, 104.267 |
| Unit cell angles | 90.00, 95.27, 90.00 |
Refinement procedure
| Resolution | 41.700 - 2.300 |
| R-factor | 0.18724 |
| Rwork | 0.185 |
| R-free | 0.23542 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ioi |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.433 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.086 | 0.397 |
| Number of reflections | 44863 | |
| <I/σ(I)> | 13.7 | 5.7 |
| Completeness [%] | 99.0 | 97.8 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 4.0M sodium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
