2ENW
Mutant Y92H structure of TTHB049 from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-01-24 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 84.974, 91.988, 110.273 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.100 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v37 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.083 | 0.334 |
Number of reflections | 25271 | |
<I/σ(I)> | 8.5 | 5.4 |
Completeness [%] | 99.8 | 99.2 |
Redundancy | 8.3 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 8.3 | 295 | 2.75M Na Chloride, 0.1M Tris, pH 8.3, Microbatch, temperature 295K |