2EMN

GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA, MUTANT

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1996-06-23
Detector	RIGAKU RAXIS II
Spacegroup name	P 61 2 2
Unit cell lengths	89.200, 89.200, 130.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.198
Rwork	0.198
R-free	0.29900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ema
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.091 *	0.310 *
Number of reflections	12729
Completeness [%]	90.9	76.3
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	20 *	protein solution is mixed in a 1:1 ratio with well solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	21 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	reservoir	ammonium sulfate	2.1 (M)
4	1	reservoir	Tris-HCl	100 (mM)