2EL7

Crystal structure of Tryptophanyl-tRNA synthetase from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-27
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	47.318, 72.488, 229.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.350 - 2.500
R-factor	0.195
Rwork	0.195
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 1MAU
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.520
High resolution limit [Å]	2.500	2.480
Number of reflections	28314
Completeness [%]	99.9	71.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Oil Batch	6.5	291	15% MPD, 0.1M cacodylate, 0.2M MgOAc, pH 6.5, Oil Batch, temperature 291K