2EL1
Structural study of Project ID PH0725 from Pyrococcus horikoshii OT3 (L44M)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-12-05 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 104.890, 104.890, 139.256 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.730 - 2.200 |
R-factor | 0.201 |
Rwork | 0.200 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.079 | 0.571 |
Number of reflections | 40227 | |
<I/σ(I)> | 10.5 | 4.48 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 13.2 | 12.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.5 | 291 | 3.85M Na Formic acid, 0.1M Acetic acid, pH 5.5, MICROBATCH, temperature 291K |