2EL0
Structural study of Project ID PH0725 from Pyrococcus horikoshii OT3 (L21M)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-01 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 105.559, 105.559, 137.448 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.280 - 2.400 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.057 | 0.134 |
| Number of reflections | 31111 | |
| <I/σ(I)> | 3.4 | 3.39 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.7 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 291 | 1.1M Li Sulfate, pH 8.27 0.1M Tris, pH 8.5, MICROBATCH, temperature 291K |
