2EKY
Crystal Structure of hypothetical protein MJ1052 from Methanocaldococcus jannaschii (Form 1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-02-05 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.437, 61.394, 87.811 |
Unit cell angles | 90.00, 108.80, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lxn |
RMSD bond length | 0.004 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.073 | 0.503 |
Number of reflections | 68040 | |
<I/σ(I)> | 9.7 | 3.3 |
Completeness [%] | 98.8 | 92.6 |
Redundancy | 5.3 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7 | 295 | 50%(v/v) PEG200, 0.1M Tris, pH 7.0, microbatch, temperature 295K |
1 | MICROBATCH | 7 | 295 | 50%(v/v) PEG200, 0.1M Tris, pH 7.0, microbatch, temperature 295K |
1 | MICROBATCH | 7 | 295 | 50%(v/v) PEG200, 0.1M Tris, pH 7.0, microbatch, temperature 295K |