2EKN

Structure of PH1811 protein from Pyrococcus horikoshii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-12-13
Detector	RIGAKU
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	119.910, 119.910, 63.960
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.050
R-factor	0.19
Rwork	0.190
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ekr
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.098
Number of reflections	33087
<I/σ(I)>	6.6
Completeness [%]	100.0	99.9
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	30% PEG 8000, 0.5M LiCl, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K