2EKM

Structure of ST1219 protein from Sulfolobus tokodaii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-12-15
Detector	RIGAKU
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	63.200, 63.200, 229.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.060
R-factor	0.18
Rwork	0.180
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2glo
RMSD bond angle	0.005
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.130
High resolution limit [Å]	2.060	2.060
Rmerge	0.096
Number of reflections	29795
<I/σ(I)>	6.8
Completeness [%]	100.0	100
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	295	28% PEG 5k, 0.2M MgSO4, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 295K