2EK9

Aminopeptidase from Aneurinibacillus sp. strain AM-1 with Bestatin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2006-08-29
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	93.813, 68.678, 77.044
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.970
R-factor	0.199
Rwork	0.199
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ek8
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.080
High resolution limit [Å]	1.970	1.970
Rmerge	0.069	0.292
Number of reflections	35820
<I/σ(I)>	18.9	4.9
Completeness [%]	99.8	100
Redundancy	4.75

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.8	299	13% PEG 8000, 0.1M MES-NaOH, 0.2M Zinc acetate, pH 5.8, VAPOR DIFFUSION, temperature 299K