2EK8

Aminopeptidase from Aneurinibacillus sp. strain AM-1

Experimental procedure

Experimental method	MAD
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2006-07-06
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	93.624, 68.618, 76.841
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.850 - 1.800
R-factor	0.213
Rwork	0.213
R-free	0.23800
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.058	0.336
Number of reflections	45919
<I/σ(I)>	31.2	6.1
Completeness [%]	98.7	97.1
Redundancy	10.6	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.8	299	13% PEG 8000, 0.1M MES-NaOH, 0.2M Zinc acetate, pH 5.8, VAPOR DIFFUSION, temperature 299K
1	VAPOR DIFFUSION	5.8	299	13% PEG 8000, 0.1M MES-NaOH, 0.2M Zinc acetate, pH 5.8, VAPOR DIFFUSION, temperature 299K