2EK2
Structural study of Project ID PH0725 from Pyrococcus horikoshii OT3 (E140M)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 104.868, 104.868, 138.745 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.720 - 2.200 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.055 | 0.273 |
| Number of reflections | 39944 | |
| <I/σ(I)> | 14.7 | 9.02 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 14 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 291 | 1.87M Na Malonic acid, 0.1M Citric acid pH 5.22, pH 6.5, MICROBATCH, temperature 291K |
