2EK1

Crystal structure of RNA-binding motif of human rna-binding protein 12

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97947, 0.97964, 0.964
Spacegroup name	P 1 21 1
Unit cell lengths	47.879, 103.227, 62.189
Unit cell angles	90.00, 91.50, 90.00

Refinement procedure

Resolution	29.760 - 2.000
R-factor	0.208
Rwork	0.208
R-free	0.27400
Structure solution method	SAD
RMSD bond length	0.028
RMSD bond angle	2.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	39769
Completeness [%]	97.1	89.8
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M Hepes pH7.5, 25% (w/v) PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 293K