2EJQ

Conserved hypothetical protein (TTHA0227) from Thermo thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-22
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	46.509, 63.281, 98.047
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.480 - 2.080
R-factor	0.235
Rwork	0.235
R-free	0.25100
Structure solution method	MAD
Starting model (for MR)	model derived from Se-MAD experiment transfered to higher resolution Native data set after single rebuilding cycle
RMSD bond length	0.009
RMSD bond angle	1.412
Data reduction software	d*TREK
Data scaling software	d*TREK (8.0SSI)
Phasing software	SOLVE (2.10)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.500	2.150
High resolution limit [Å]	2.080	2.080
Rmerge	0.048	0.305
Total number of observations		11770
Number of reflections	17938
<I/σ(I)>	21.8	4.6
Completeness [%]	99.6	99.9
Redundancy	6.85	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	1.2M Ammonium tartrate, 0.1M Bis-Tris Propane, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	1.2M Ammonium tartrate, 0.1M Bis-Tris Propane, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	1.2M Ammonium tartrate, 0.1M Bis-Tris Propane, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K