2EJB
Crystal Structure Of Phenylacrylic Acid Decarboxylase from Aquifex aeolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 130.934, 130.934, 130.934 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.730 - 2.150 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sbz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.730 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.048 | 0.309 |
| Number of reflections | 9696 | |
| <I/σ(I)> | 36.8 | 4.8 |
| Completeness [%] | 94.9 | 95.4 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.5 | 295 | 10% PEG 6000, 2M NaCl, pH 4.5, microbatch, temperature 295K |
