2EJ9
Crystal Structure Of Biotin Protein Ligase From Methanococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.114, 61.843, 42.438 |
| Unit cell angles | 90.00, 96.74, 90.00 |
Refinement procedure
| Resolution | 24.930 - 2.000 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wq7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.930 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.059 | 0.418 |
| Number of reflections | 20166 | |
| <I/σ(I)> | 21.3 | 5.1 |
| Completeness [%] | 98.6 | 96.4 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 295 | 20w/v(%) PEG 10000, 20w/v(%) Ethylene glycol 20w/v, 0.1M HEPES, pH 7.5, microbatch, temperature 295K |
