2EJ8
Crystal structure of APPL1 PTB domain at 1.8A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.230, 63.566, 52.449 |
| Unit cell angles | 90.00, 102.67, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.840 |
| R-factor | 0.19526 |
| Rwork | 0.193 |
| R-free | 0.23014 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.438 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.104 | 0.455 |
| Number of reflections | 24816 | |
| <I/σ(I)> | 9.59801 | 2.73783 |
| Completeness [%] | 97.3 | 91.4 |
| Redundancy | 3.5652 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 100mM Na Citrate, 10% PEG 3350, 15% Isopropanol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
