2EIX

The Structure of Physarum polycephalum cytochrome b5 reductase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-01
Detector	Bruker DIP-6040
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	101.084, 136.369, 45.718
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.355 - 1.560
Rwork	0.180
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1umk
RMSD bond length	0.020
RMSD bond angle	2.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.355	1.620
High resolution limit [Å]	1.560	1.560
Number of reflections	83782
<I/σ(I)>	28.5	3.7
Redundancy	7.1	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	20% PEG 4000, 50mM ADA, 200mM Sodium iodide, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K