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2EIW

Crystal analysis of delta1-pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus with bound L-proline

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL44B2
Synchrotron siteSPring-8
BeamlineBL44B2
Temperature [K]100
Detector technologyCCD
Collection date2006-11-25
DetectorADSC QUANTUM 210
Wavelength(s)1.0
Spacegroup nameH 3
Unit cell lengths100.349, 100.349, 279.993
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution24.610 - 1.900
R-factor0.15868
Rwork0.157
R-free0.19157
Structure solution methodFOURIER SYNTHESIS
Starting model (for MR)2bhq
RMSD bond length0.014
RMSD bond angle1.377
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareREFMAC
Refinement softwareREFMAC (5.2.0005)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]25.0001.970
High resolution limit [Å]1.9001.900
Rmerge0.0690.306
Number of reflections82759
<I/σ(I)>24.45.2
Completeness [%]100.0100
Redundancy5.75.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP5.229330% MPD, 50MM SODIUM CITRATE/HCL, PH5.2; THEN SOAKED IN 30% MPD, 50mM L-proline, 50MM SODIUM Acetate, PH5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K

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