Experimental procedure
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 110.200, 110.200, 156.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.23400 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.400 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.200 | 2.200 * |
| Rmerge | 0.082 | |
| Total number of observations | 140425 * | |
| Number of reflections | 16178 | |
| Completeness [%] | 86.5 | 72 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | reservoir | HEPES | 0.1 (M) | |
| 5 | 1 | reservoir | sodium citrate | 0.75 (M) | |
| 6 | 1 | reservoir | 4 (mM) |
