2EIG
Lotus tetragonolobus seed lectin (Isoform)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-01 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.43 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.892, 65.835, 102.537 |
| Unit cell angles | 90.00, 92.00, 90.00 |
Refinement procedure
| Resolution | 34.460 - 2.000 |
| R-factor | 0.1914 |
| Rwork | 0.188 |
| R-free | 0.25083 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.201 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.420 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.095 | 0.459 |
| Number of reflections | 61847 | |
| <I/σ(I)> | 10.8 | 3.5 |
| Completeness [%] | 99.4 | |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 8% PEG 3350, 20% Isopropanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
