2EIE

Crystal Structure of Galactose Oxidase complexed with Azide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.864
Spacegroup name	P 21 21 21
Unit cell lengths	59.309, 89.294, 134.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.000 - 1.800
Rwork	0.192
R-free	0.20900
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.012
RMSD bond angle	1.565
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	39.100
High resolution limit [Å]	1.800
Rmerge	0.047
Number of reflections	65987
Completeness [%]	97.0
Redundancy	10.33

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	298	13.5% PEG 8000, 200mM calcium acetate, 100mM MES pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K