2EIB

Crystal Structure of Galactose Oxidase, W290H mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Detector	SIEMENS-NICOLET X100
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	98.000, 89.400, 86.700
Unit cell angles	90.00, 117.80, 90.00

Refinement procedure

Resolution	10.000 - 2.100
Rwork	0.157
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1gog
RMSD bond length	0.017
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	298	1-2M Amonium Sulphate, 0.1M sodium acetate pH 4-5, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K