2EIA

X-RAY CRYSTAL STRUCTURE OF EQUINE INFECTIOUS ANEMIA VIRUS (EIAV) CAPSID PROTEIN P26

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	1997-11-11
Detector	BRANDEIS
Wavelength(s)	1.0333, 1.07168, 1.07202
Spacegroup name	P 61 2 2
Unit cell lengths	100.480, 100.480, 157.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.000 - 2.700
R-factor	0.236
Rwork	0.236
R-free	0.29400
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	18.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.061 *	0.326 *
Total number of observations	246510 *
Number of reflections	13524
<I/σ(I)>	15	3
Completeness [%]	99.7	97.7
Redundancy	20	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		0.1M SODIUM CITRATE, 10% PEG 3350, 15% ISOPROPANOL, PH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	22 (mg/ml)
2	1	drop	sodium phosphate	1 (mM)
3	1	reservoir	sodium citrate	0.1 (M)
4	1	reservoir	PEG3350	10 (%(w/v))
5	1	reservoir	isopropanol	15 (%(v/v))