2EHW

Conserved hypothetical proteim (TTHB059) from Thermo thermophilus HB8

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-22
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97915, 0.97952, 1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	58.450, 132.460, 117.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.210 - 2.220
Rwork	0.220
R-free	0.28400
Structure solution method	MAD
RMSD bond angle	0.006
Data reduction software	d*TREK
Data scaling software	d*TREK (8.0SSI)
Phasing software	SOLVE (2.10)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.220	2.300
High resolution limit [Å]	2.220	2.220
Rmerge	0.076	0.341
Total number of observations		16348
Number of reflections	22728
<I/σ(I)>	14.5	5.5
Completeness [%]	99.0	99.1
Redundancy	7.12	7.27

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	23% PEG MME 2000, 20mM Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	8	293	23% PEG MME 2000, 20mM Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	8	293	23% PEG MME 2000, 20mM Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K